Sugars released from (WOP) were fermented into bioethanol (0.498 g bioethanol/ g glucose) with Saccharomyces cerevisiae. Under optimum hydrolysis conditions the maximum amount of sugar released and hydrolysis efficiency were (23.86 g/L) and (89.4 %), respectively. Two of three factors (hydrolysis time and temperature) were identified to have significant effects (p < 0.05) on glucose release.

The effects of three factors (hydrolysis time, temperature and pH) on the extent of saccharification were optimized by means of central composite design (CCD) and response surface methodology (RSM). Hydrolysis process was conducted with a crude cellulolytic enzymes produced by Cellulomonas uda (PTCC 1259). The pretreatment was accomplished by first presoaking of (WOP) for (24 h), followed by treatment with a 0.1% N NaOH solution. The potential of waste office paper (WOP) to be used as renewable biomass for the production of reducing sugar via the enzymatic hydrolysis was evaluated in this study, as well as the production of bioethanol. Sugars released from waste paper were subsequently converted into ethanol (0.38 g ethanol g(-1) sugar) with Saccharomyces cerevisiae CTM-30101.īioethanol produced from lignocellulosic feedstock is a renewable substitute to lessening fossil fuels. Under optimal hydrolysis conditions, maximum degrees of saccharification of newspaper and office paper were 67 and 92 %, respectively. The effects of three factors (dry matter quantity, phosphoric acid pretreatment and hydrolysis time) on the extent of saccharification were also assessed and quantified by using a methodical approach based on response surface methodology. Surfactant pretreatment effect on waste paper enzymatic digestibility was studied and Triton X-100 at 0.5 % (w w(-1)) has improved the digestibility of newspaper about 45 %. Hydrolysis step was conducted with a mixture of cellulolytic enzymes produced locally by Trichoderma reesei Rut-C30 (cellulase-overproducing mutant) and Aspergillus niger F38 cultures. Two types of waste paper materials, newspaper and office paper, were evaluated for their potential to be used as a renewable feedstock for the production of fermentable sugars via enzymatic hydrolysis of their cellulose fractions. In this study, the feasibility of ethanol production from waste paper using the separate hydrolysis and fermentation (SHF) was investigated. Ethanol produced from lignocellulosic biomass is a renewable alternative to diminishing petroleum-based liquid fuels.

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